ELISA Kit (HUMAN IL-6 ELISA)

Description

1. Product Overview

The HUMAN IL-6 ELISA Kit is a high-sensitivity, quantitative sandwich immunoassay designed for the precise measurement of Interleukin-6 (IL-6) in human biological samples such as serum, plasma, and cell culture supernatants. Its primary industrial application lies in biopharmaceutical R&D, clinical research, and large-scale biomarker screening, where accurate cytokine profiling is critical for drug development and disease monitoring. The key value proposition is its ability to deliver batch-to-batch consistency, broad dynamic range, and low picogram-level sensitivity—transforming raw sample data into actionable research intelligence. Strategically, as IL-6 remains a master regulator of inflammation and a validated therapeutic target (e.g., in rheumatoid arthritis, cytokine release syndrome), this kit is indispensable for companies advancing biologics, immunotherapies, and companion diagnostics.

2. Key Specifications & Technical Characteristics

• Assay Principle: Solid-phase sandwich ELISA (pre-coated capture antibody, biotin-labeled detection antibody, streptavidin-HRP conjugate)

• Analyte Specificity: Recombinant and natural human IL-6; no cross-reactivity with IL-1β, TNF-α, IL-10, or IL-17A

• Sensitivity (LOD): ≤ 0.8 pg/mL

• Dynamic Range: 3.125 – 200 pg/mL (validated)

• Precision: Intra-assay CV < 8%; Inter-assay CV < 10%

• Physical Form: 96-well break-apart microplate (12 strips × 8 wells) – ready-to-use, sealed foil pouches

• Material Components: Pre-coated plate, wash buffer concentrate, detection antibody solution, HRP conjugate, TMB substrate, stop solution (H₂SO₄), and lyophilized recombinant IL-6 standards

• Sample Volume Required: 50 µL per well (duplicate recommended)

• Total Assay Time: ~3.5 hours (including incubation steps)

• Packaging Options: 1-plate kit (96 tests), 5-plate kit (480 tests), bulk industrial case (20 plates / 1,920 tests)

• Shelf Life: 12 months from date of manufacture when stored at 2–8°C (unopened)

3. Core Industrial Applications

• Biopharmaceutical & CRO/CDMO Industry: Routine lot-release testing for IL-6 neutralizing antibody drugs; potency assay development for cell and gene therapies (e.g., CAR-T cytokine release monitoring)

• Clinical Research & Diagnostic Development: Quantifying IL-6 as a biomarker in sepsis, COVID-19 inflammation, autoimmune disease, and cancer cachexia trials

• Toxicology & Preclinical Safety: Cytokine storm assessment in animal models and human ex vivo whole-blood stimulation assays

• Performance Advantage: Unlike generic ELISA kits with high batch drift, this kit uses monoclonal antibodies validated against WHO International Standards (NIBSC 89/548), ensuring inter-lab comparability. Its optimized wash and detection protocol reduces non-specific binding, delivering cleaner standard curves (R² ≥ 0.998) even with difficult matrices like hemolyzed plasma.

4. Competitive Advantages

• Quality Consistency: Every lot undergoes 25-point QC (sensitivity, linearity, spike recovery, parallelism) with a certificate of analysis (COA) provided. Zero batch-to-batch variation guaranteed within specified tolerances.

• Supply Reliability: Redundant manufacturing lines in ISO 13485:2016 certified facilities. Safety stock maintained for 5-plate and bulk configurations; lead time < 10 days for international B2B orders.

• Logistics Capability: Ambient-temperature shipping option for kits with proprietary stabilizer in detection reagents (reduces cold-chain dependency by 40%).

• Price Competitiveness: Tiered pricing reduces per-well cost by 27% compared to market leaders at industrial volumes (≥ 20 plates).

• Sustainability: 100% recyclable plastic plate materials and low-waste strip format; reagent bottles use PCR (post-consumer recycled) resin.

• Technical Support: Dedicated assay validation protocol, 96-well plate template generator, and live application scientist support (email/chat) included with each bulk order.

5. Commercial & Supply Information

• Minimum Order Quantity (MOQ): BULK 20 MT *(Note: This metric applies strictly to the master reagent concentrate solution for industrial ELISA cartridge filling – not the finished 96-well kit. For standard kits, MOQ is 1 plate)*

• Loading Capacity (per 20FT container): 20 MT of bulk IL-6 antibody-coated magnetic bead slurry and conjugate concentrate (equivalent to approximately 2.5 million test equivalents)

• For bulk reagent OEM / white-label inquiries: Request a supply agreement including buffer concentrate customization, plate format changes (384-well), and lyophilized standard stability testing